mouse monoclonal anti nka α subunit antibody  (Developmental Studies Hybridoma Bank)

Journal: Science Advances

Article Title: The mechanism of action of digoxin requires the sodium-dependent inactivation of the sodium-calcium exchanger

doi: 10.1126/sciadv.ady9596

Figure Lengend Snippet: ( A ) Representative Western blot of NKA α-subunit (top) and GAPDH (bottom) expression of WT and K229Q ventricular homogenates. ( B ) Summary densitometry analysis showing the fold change in NKA protein expression between WT and K229Q mice, represented as NKA intensity/GAPDH intensity, normalized to WT levels. Hearts: WT, n = 10; K229Q, n = 10. Immunofluorescence image of WT ( C ) and K229Q ( D ) adult ventricular myocytes. Cells were stained with anti-NKA α-subunit antibody. The panel shows the magnified area of the cell. Cells in the image are maximum intensity projections. Scale bar, 10 μm. ( E ) NKA fluorescence is reported as corrected total cell fluorescence (CTCF) divided by cell area (CTCF/area). Each point represents one animal. Cells (four to six) were averaged for each animal. Animal averages were used for statistical comparisons. ( F ) Data from each individual cell, as in (E). NKA fluorescence was unchanged in K229Q myocytes compared to WT. Data are means ± SEM. Cells/animals: WT, n = 15/3; K229Q, n = 15/3.

Article Snippet: Myocytes were then incubated with the mouse monoclonal anti-NKA α-subunit antibody (1:200, a5, DSHB) in 3% BSA PBS overnight at 4°C.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Fluorescence

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP1, 2, and 6 in whole body adult female Ae. aegypti. Immunohistochemical localization of water selective AaAQPs (red staining) in female Ae. aegypti mosquitoes (~10–12 days old). Each AaAQP (red staining) was localized under non-blood fed conditions, 0.5hr post blood meal conditions, and 24hr post blood meal conditions. Whole body sections of the abdominal segment immunolocalizing AaAQP1 in (A–C) , AaAQP2 in (D–F) , and AaAQP6 in (G–I) . In (B) , an inset image (B’) shows AaAQP1 membrane localization in the HG. In (E) , an inset image (E’) shows AaAQP2 membrane localization in the MG. All images were taken at 10x magnification (n=3). The Na + /K + -ATPase (NKA) was used as a membrane marker (green staining) and nuclei were stained with DAPI (blue). MT, Malpighian tubules; FB, fat body; OV, ovaries; HG, hindgut; MG, midgut; RP, rectal pads. White arrows indicate aggregated staining of AaAQP in the MTs.

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Membrane, Marker

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP4 and 5 in whole body adult female Ae. aegypti. Immunohistochemical localization of entomoglyceroporin AaAQPs (red staining) in female Ae. aegypti mosquitoes (~10–12 days old). AaAQP4 and 5 (red staining) were localized under non-blood fed conditions, 0.5hr post blood meal conditions, and 24hr post blood meal conditions. Whole body sections of the abdominal segment immunolocalizing AaAQP4 in (A–C) with basolateral membrane of MTs encircled in dashed line and AaAQP5 in (D–F) . All images were taken at 10x magnification (n=3). The Na + /K + -ATPase (NKA) was used as a membrane marker (green staining) and nuclei were stained with DAPI (blue). MT, Malpighian tubules; FB, fat body; OV, ovaries; HG, hindgut; MG, midgut; RP, rectal pads. White arrows indicate aggregated staining of AaAQP in the MTs.

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Membrane, Marker

Journal: Frontiers in Insect Science

Article Title: Protein localization of aquaporins in the adult female disease vector mosquito, Aedes aegypti

doi: 10.3389/finsc.2024.1365651

Figure Lengend Snippet: Localization of AaAQP1 in Malpighian tubules of adult female Ae. aegypti. Immunohistochemical localization of AaAQP1 (red staining) in isolated Malpighian tubules (MTs) of female Ae. aegypti (~10–12 days old) MTs were isolated from non-blood fed mosquitoes, and mosquitoes that were fed blood either 0.5hr or 24hr prior. (A–C) shows localization of AaAQP1 at the apical membrane of the MTs. White arrows indicate the apical membrane of the MTs. (D–F) shows localization of AaAQP1 at the apical membrane of the MTs, merged with staining observed for V-type H + -ATPase (VA), which was used as an apical membrane marker (green staining). Co-localization of AaAQP1 with VA appears yellow/orange in colour. (G–I) shows brightfield images of MT sections, which was used to identify apical and basolateral membranes. All images were taken at 20x magnification (n=4) and nuclei were stained with DAPI (blue).

Article Snippet: WB tissue sections were also probed with a mouse monoclonal anti-a5 antibody for Na+/K+-ATPase (NKA) (Douglas Fambrough, Developmental Studies Hybridoma Bank, IA, USA, 1:10 dilution) as a membrane marker.

Techniques: Immunohistochemical staining, Staining, Isolation, Membrane, Marker